![]() Caution: Not intended for human or animal diagnostic or therapeutic uses. Note: The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards: supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. Compare and view all other protein standards and ladders Applications Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Migration patterns of BLUelf prestained protein ladder in different electrophoresis conditions are listed below: The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. G uide for Molecular Weight Estimation (kDa) Under suggested conditions, BLUelf prestained protein ladder resolves 10 major bands in 4-12% Bis-Tris Gel (MES buffer) and after Western blotting to nitrocellulose membrane. Do not heat, dilute, add reducing agent before loading.Īpproximately 0.1-0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2% SDS, 10 mM Dihiothreltol, 3.6 M Urea, and 15% (v/v) (Glycerol). The ladder is supplied in gel loading buffer and is ready to use. The BLUelf prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). The BLUltra prestained protein ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights from 6.5 to 270 kDa. Apply more for thicker (>1.5mm) or larger gel. 1.5-2.5μl per well for general Western transferring Note: The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.- 3μl or 5μl per loading for clear visualization during electrophoresison 15-well or 10-well mini-gel, respectively. Sizing of proteins on SDS-polyacrylamide gels and western blots.Monitoring protein transfer onto membranes after western blotting.Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.Membrane-compatible-colored bands transfer to membranes for Western blotting.Two reference bands-orange at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa.Quality tested-each lot evaluated by SDS-PAGE and Western blotting.Sharp bands-color-coded bands of similar intensity for easy visualization.Ready-to-use-supplied in a loading buffer for direct loading on gels no need to boil.Size range-10 proteins spanning from 10 to 180 kDa.The ladder contains one orange reference band at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa. This prestained protein marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. ABP Prestained Protein Marker is a mixture of ten blue, orange or green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein marker is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.
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